|
KMID : 0385219940040010008
|
|
Korean Journal of Gerontology
1994 Volume.4 No. 1 p.8 ~ p.13
|
|
|
Change in Prooxidant Activity in Rat Lung Cytosol with Age
|
|
Choi Kee-Oh
Wee Jung-Soon
Song Dong-Up
Joung Young-Do
Jeong Yu-Jeong
Lee Min-Hwa
|
|
|
Abstract
|
|
|
|
We have observed that rat lung cytosol caused and oxidative inactivation of exogenous glutamine synthetase(GS), and metal-catalyzed oxidation system(s) in the small-molecular fraction of cytosol and a prooxidant in the large-molecular fraction which simulates metal-catalyzed oxidation are suggested to be involved in the enzyme inactivation process. In this study, the difference in the oxidant activity of lung cytosol between 1 month- and 6 month-old rats(N=10) was investigated using the GS inactivation assay. Lung sytosol from 6 month-old group inactivated GS more rapidly than that from 1 month-old group. Small-molecular(Mr<∼10,000)fraction of cytosol from both the age groups inactivated GS at the similar rates. However, there was a significant difference between two age groups in the prooxidant activity of large-molecular(Mr>∼10,000)fraction of cytosol which stimulated the small-molecular fraction-induced GS inactivation. Oxidant activity of rat skin cytosol was also investigated for the above two age groups(N=5). Skin cytosol from 6 month-old group inactivated GS more rapidly than that from 1 month-old group. The above results suggest that oxidant activity increases with age in the rat lung and other tissues, and that the increase in oxidant activity may involve a prooxidant protein present in cytosol.
|
|
|
KEYWORD
|
|
|
Age-related changes, Oxidant activity, Prooxidant, Rat lung, Skin
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|